The 68-year-old from Toowoomba contracted COVID-19 on board a Royal Caribbean cruise ship that docked in Sydney last week.
Garry Kirstenfield was in the intensive care unit in Toowoomba Hospital and died this afternoon.
His wife is in self-isolation along with all of his immediate family.
His family have described him as “kind and adorable”.
A Queensland woman last week died from the virus in Sydney after a flight from the Sunshine Coast.
Kirstenfield is the ninth person to die a coronavirus-related death in Australia.
A Queensland Health spokesperson said in a statement Kirstenfield had a “serious underlying medical condition before contracting the virus”.
It added: “Queensland Health offers it’s sincere condolences to his family.”